Characterization of the ethanol-inducible alc gene-expression system in Arabidopsis thaliana

Citation
Ha. Roslan et al., Characterization of the ethanol-inducible alc gene-expression system in Arabidopsis thaliana, PLANT J, 28(2), 2001, pp. 225-235
Citations number
35
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences","Animal & Plant Sciences
Journal title
PLANT JOURNAL
ISSN journal
0960-7412 → ACNP
Volume
28
Issue
2
Year of publication
2001
Pages
225 - 235
Database
ISI
SICI code
0960-7412(200110)28:2<225:COTEAG>2.0.ZU;2-D
Abstract
Controlled expression of transgenes in plants is key to the characterizatio n of gene function and the regulated manipulation of growth and development . The alc gene-expression system, derived from the filamentous fungus Asper gillus nidulans, has previously been used successfully in both tobacco and potato, and has potential for use in agriculture. Its value to fundamental research is largely dependent on its utility in Arabidopsis thaliana. We ha ve undertaken a detailed function analysis of the ak regulon in A. thaliana . By linking the alcA promoter to beta -glucuronidase (GUS), luciferase (LU C) and green fluorescent protein (GFP) genes, we demonstrate that alcR-medi ated expression occurs throughout the plant in a highly responsive manner. Induction occurs within one hour and is dose-dependent, with negligible act ivity in the absence of the exogenous inducer for soil-grown plants. Direct application of ethanol or exposure of whole plants to ethanol vapour are e qually effective means of induction. Maximal expression using soil-grown pl ants occurred after 5 days of induction. In the majority of transgenics, ex pression is tightly regulated and reversible. We describe optimal strategie s for utilizing the ak system in A. thaliana.