Wolves (Canis lupus) were captured in three areas of Interior Alaska (USA).
Four hundred twenty-five sera were tested for evidence of exposure to cani
ne coronavirus by means of an indirect fluorescent antibody procedure. Seru
m antibody prevalence averaged 70% (167/240) during the spring collection p
eriod and 25% (46/185) during the autumn collection period. Prevalence was
0% (0/42) in the autumn pup cohort (age 4-5 mo), and 60% (58/97) in the spr
ing pup cohort (age 9-10 mo). Prevalence was lowest in the Eastern Interior
study area. A statistical model indicates that prevalence increased slight
ly each year in all three study areas, These results indicate that transmis
sion occurs primarily during the winter months, antibody decay is quite rap
id, and reexposure during the summer is rare.