Isolation of a homodimeric lectin with antifungal and antiviral activitiesfrom red kidney bean (Phaseolus vulgaris) seeds

Ye, XY Ng, TB Tsang, PWK Wang, J

Xy. Ye et al., Isolation of a homodimeric lectin with antifungal and antiviral activitiesfrom red kidney bean (Phaseolus vulgaris) seeds, J PROTEIN C, 20(5), 2001, pp. 367-375

32

INGLESE

Article

Biochemistry & Biophysics

JOURNAL OF PROTEIN CHEMISTRY

0277-8033 → ACNP

20

5

2001

367 - 375

ISI

0277-8033(200107)20:5<367:IOAHLW>2.0.ZU;2-W

A homodimeric lectin adsorbed on Affi-gel blue gel and CM-Sepharose and pos sessing a molecular weight of 67 kDa was isolated from red kidney beans. Th e hemagglutinating activity of this lectin was inhibited by glycoproteins b ut not by simple sugars. The lectin manifested inhibitory activity on human immunodeficiency virus-1 reverse transcriptase and alpha -glucosidase. The N-terminal sequence of the lectin exhibited some differences from previous ly reported lectins from Phaseolus vulgaris but showed some similarity to c hitinases. It exerted a suppressive effect on growth of the fungal species Fusarium oxysporum, Coprinus comatus, and Rhizoctonia solani. The lectin ha d low ribonuclease and negligible translation-inhibitory activities.