Isolation of unguilin, a cyclophilin-like protein with anti-mitogenic, antiviral, and antifungal activities, from black-eyed pea

Authors
Citation
Xy. Ye et Tb. Ng, Isolation of unguilin, a cyclophilin-like protein with anti-mitogenic, antiviral, and antifungal activities, from black-eyed pea, J PROTEIN C, 20(5), 2001, pp. 353-359
Citations number
28
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
JOURNAL OF PROTEIN CHEMISTRY
ISSN journal
0277-8033 → ACNP
Volume
20
Issue
5
Year of publication
2001
Pages
353 - 359
Database
ISI
SICI code
0277-8033(200107)20:5<353:IOUACP>2.0.ZU;2-X
Abstract
A protein designated unguilin was isolated from seeds of the black-eyed pea (Vigna unguiculata). It possesses a molecular weight of 18 kDa and an N-te rminal sequence resembling that of cyclophilins and the cyclophilin-like an tifungal protein from mung beans, and was adsorbed on Affi-gel blue gel and CM-Sepharose. Unguilin exerted an antifungal effect toward fungi including Coprinus comatus, Mycosphaerella arachidicola, and Botrytis cinerea. In ad dition, unguilin was capable of inhibiting human immunodeficiency virus-1 r everse transcriptase and the glycohydrolases alpha- and beta -glucosidases which are involved in HIV infection. Unguilin was devoid of lectin and ribo nuclease activities. It inhibited methyl-H-3-thymidine uptake by mouse sple nocytes and it weakly inhibited translation in a rabbit reticulocyte lysate system. Unguilin resembles mungin in some aspects, but differs from it in others.