Isolation of unguilin, a cyclophilin-like protein with anti-mitogenic, antiviral, and antifungal activities, from black-eyed pea

Ye, XY Ng, TB

Xy. Ye et Tb. Ng, Isolation of unguilin, a cyclophilin-like protein with anti-mitogenic, antiviral, and antifungal activities, from black-eyed pea, J PROTEIN C, 20(5), 2001, pp. 353-359

28

INGLESE

Article

Biochemistry & Biophysics

JOURNAL OF PROTEIN CHEMISTRY

0277-8033 → ACNP

20

5

2001

353 - 359

ISI

0277-8033(200107)20:5<353:IOUACP>2.0.ZU;2-X

A protein designated unguilin was isolated from seeds of the black-eyed pea (Vigna unguiculata). It possesses a molecular weight of 18 kDa and an N-te rminal sequence resembling that of cyclophilins and the cyclophilin-like an tifungal protein from mung beans, and was adsorbed on Affi-gel blue gel and CM-Sepharose. Unguilin exerted an antifungal effect toward fungi including Coprinus comatus, Mycosphaerella arachidicola, and Botrytis cinerea. In ad dition, unguilin was capable of inhibiting human immunodeficiency virus-1 r everse transcriptase and the glycohydrolases alpha- and beta -glucosidases which are involved in HIV infection. Unguilin was devoid of lectin and ribo nuclease activities. It inhibited methyl-H-3-thymidine uptake by mouse sple nocytes and it weakly inhibited translation in a rabbit reticulocyte lysate system. Unguilin resembles mungin in some aspects, but differs from it in others.