Mutagenicity evaluation of forty-one metal salts by the umu test

Citation
A. Yamamoto et al., Mutagenicity evaluation of forty-one metal salts by the umu test, J BIOMED MR, 59(1), 2002, pp. 176-183
Citations number
33
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Multidisciplinary
Journal title
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
ISSN journal
0021-9304 → ACNP
Volume
59
Issue
1
Year of publication
2002
Pages
176 - 183
Database
ISI
SICI code
0021-9304(200201)59:1<176:MEOFMS>2.0.ZU;2-J
Abstract
Metallic biomaterials implanted in a human body may corrode and wear, relea sing metal ions and debris which may induce adverse reactions such as infla mmation, allergy, neoplastic formation, developmental malformation, etc. Mu tagenicity is a very fundamental and important toxicity related to carcinog enicity and reproductive/developmental toxicity because the damages to gene s or DNA can be a cause of carcinogenesis and developmental abnormalities. However, available mutagenic data on metallic ions and compounds are restri cted to the number of elements. Therefore, to obtain the systematic data ne cessary for metal ion mutagenicity, 41 metal salts encompassing 36 metals a nd 5 metallic elements tested with different valences, were evaluated on th eir mutagenicity by a microbial test, the umu test. As a result, K2Cr2O7, R hCl3, IrCl4, and MgCl2 are positive without metabolic activation. Concentra tions having the maximum mutagenic effect (C-max) are 9.65 x 10(-5), 1.00 X 10(-4), 3.11 x 10(-3), 4.12 x 10(-3) mol(.)L(-1), respectively. CuCl2, VCl 3, CuCl, RhCl3, K2Cr2O7, and IrCl4 are positive with metabolic activation b y S-9 mix with C-max of 1.60 x 10(-5), 3.91 x 10(-5), 1.57 x 10(-4), 2.00 x 10(-4) 3.86 x 10(-4), 1.56 x 10(-2) mol(.)L(-1), respectively. Thirty-five metal salts were negative for tests performed both with and without metabo lic activation, whereas it was impossible to evaluate the mutagenicity of M oCl5 and ZrCl4 by the umu test because of their colorimetric reaction to te sting reagents. (C) 2001 John Wiley & Sons, Inc.