PCR amplification of 55 strains of Enterobacter cloacae indicated 51 of the
m had amp C structural gene verified by DNA sequence and Southern blotting.
All PCR products were cleaved into 666- and 328-bp fragments by Kpn1 restr
iction enzyme. Imipenem was the most potent inducer for mRNA expression of
amp C gene and beta -lactamase activity. The beta -Lactamase inhibitor R048
1220 strongly inhibited Amp C beta -lactamases, 96.4% (53/55) of Enterobact
er cloacae producing Amp C enzyme were susceptible to cefepime. (C) 2001 El
sevier Science B.V. and International Society of Chemotherapy. All rights r