Isolation and expression of an ecdysteroid-inducible neutral endopeptidase24.11-like gene in wing discs of Bombyx mori

Citation
X. Zhao et al., Isolation and expression of an ecdysteroid-inducible neutral endopeptidase24.11-like gene in wing discs of Bombyx mori, INSEC BIO M, 31(12), 2001, pp. 1213-1219
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Entomology/Pest Control","Biochemistry & Biophysics
Journal title
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY
ISSN journal
0965-1748 → ACNP
Volume
31
Issue
12
Year of publication
2001
Pages
1213 - 1219
Database
ISI
SICI code
0965-1748(20011101)31:12<1213:IAEOAE>2.0.ZU;2-I
Abstract
In the process of comparison of two cDNA libraries (W0, W2), we isolated a clone from the wing discs of Bombyx mori encoding a putative neutral endope ptidase 24.11-like gene. The predicted open reading frame encoded 772 amino acid residues, having about 53% identity with Drosophila GH07643, 36% with rat NEP, and 34% with rat ECE. This is the first NEP gene isolated in inve rtebrate. A 3.6-kb transcript was found to accumulate in the wing disc acco rding to the increase of ecdysteroid titer during metamorphosis. Accumulati on of the transcript was induced in wing discs with 20-hydroxyecdysone abou t 20h after incubation, which was inhibited by cycloheximide. This gene is ecdysone-inducible, appears to encode a functional protein, and may functio n during wing metamorphosis. (C) 2001 Elsevier Science Ltd. All rights rese rved.