Identification, genomic organization, and mRNA expression of LACTB, encoding a serine beta-lactamase-like protein with an amino-terminal transmembrane domain

Citation
Ts. Smith et al., Identification, genomic organization, and mRNA expression of LACTB, encoding a serine beta-lactamase-like protein with an amino-terminal transmembrane domain, GENOMICS, 78(1-2), 2001, pp. 12-14
Citations number
4
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
GENOMICS
ISSN journal
0888-7543 → ACNP
Volume
78
Issue
1-2
Year of publication
2001
Pages
12 - 14
Database
ISI
SICI code
0888-7543(200111)78:1-2<12:IGOAME>2.0.ZU;2-8
Abstract
Database searching with bacterial serine beta -lactamases identified mouse expressed sequence tags (ESTs) with significant similarity scores. The clon ed mouse cDNA encodes a novel 551-amino-acid protein, LACTB, with a predict ed amino-terminal transmembrane domain but no signal peptide. It contains a n active site motif related to C-class P-lactamases. Homologues were detect ed in sequence data from human, rat, cow, rabbit, pig, toad, zebrafish, and Caenorhabditis elegans, but not in Saccharomyces cerevisae or Drosophila m elanogaster. The genes were mapped to human chromosome 15q22.1 and mouse ch romosome 9. Sequencing of a 14.7-kb fragment of mouse genomic DNA defined s ix exons. A virtual human cDNA and a 549-residue protein, predicted from un finished genomic sequence, showed the same intron/exon structure. Northern blot analysis showed expression of the 2.3-kb mRNA predominantly in mouse l iver and human skeletal muscle. This is the first reported vertebrate examp le of this microbial peptidase family.