Hepatoprotective effects of Platycodon grandiflorum on acetaminophen-induced liver damage in mice

Citation
Kj. Lee et al., Hepatoprotective effects of Platycodon grandiflorum on acetaminophen-induced liver damage in mice, CANCER LETT, 174(1), 2001, pp. 73-81
Citations number
36
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
CANCER LETTERS
ISSN journal
0304-3835 → ACNP
Volume
174
Issue
1
Year of publication
2001
Pages
73 - 81
Database
ISI
SICI code
0304-3835(200112)174:1<73:HEOPGO>2.0.ZU;2-Z
Abstract
The protective effects of an aqueous extract from the roots of Platycodon g randiforum A. DC (Campanulaceae), Changkil (CK), on acetaminophen (APAP)-in duced hepatotoxicities and the possible protective mechanisms involved were investigated in mice. Pretreatment with CK prior to the administration of APAP significantly prevented the increase in serum alanine aminotransferase and aspartate aminotransferase activity and hepatic lipid peroxidation in a dose-dependent manner. APAP-induced hepatotoxicity was also essentially p revented as evidenced by liver histopathology. Hepatic glutathione levels a nd glutathione-S-transferase activities were not affected by treatment with CK alone, but pretreatment with CK protected the APAP-induced depletion of hepatic glutathione levels. The effects of CK on cytochrome P450 (P450) 1A 2 and 2E1, the major isozymes involved in APAP bioactivation, were investig ated. In microsomal incubations, CK effectively inhibited P450 1A2-dependen t methoxyresorufin O-deethylase activities and the P450 2E1-dependent p-nit rophenol and aniline hydroxylase. The results suggest that the protective e ffects of CK against the APAP-induced hepatotoxicity may, at least in part, be due to its ability to block P450-mediated APAP bioactivation. (C) 2001 Elsevier Science Ireland Ltd. All rights reserved.