S. Zhang et al., Development of a quantitative relationship between inhibition percentage and both incubation time and inhibitor concentration for inhibition biosensors - theoretical and practical considerations, BIOSENS BIO, 16(9-12), 2001, pp. 1119-1126
Theoretical and practical insights into the design and development of immob
ilised enzyme inhibition biosensors are reported. A general mathematical ex
pression relating the percent of enzyme inhibition (i.e. the analytical sig
nal) to both the inhibitor concentration and the incubation time is present
ed. The relevant physical, chemical and biochemical parameters required by
the model are developed and discussed in terms of the inhibition of acetylc
holinesterase by the organophosphorous pesticide, paraoxon. A second enzyme
, choline oxidase and an amperometric transducer are used to facilitate the
determination acetylcholinesterase inhibitor. (C) 2001 Elsevier Science B.
V. All rights reserved.