Development of a quantitative relationship between inhibition percentage and both incubation time and inhibitor concentration for inhibition biosensors - theoretical and practical considerations

Citation
S. Zhang et al., Development of a quantitative relationship between inhibition percentage and both incubation time and inhibitor concentration for inhibition biosensors - theoretical and practical considerations, BIOSENS BIO, 16(9-12), 2001, pp. 1119-1126
Citations number
25
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOSENSORS & BIOELECTRONICS
ISSN journal
0956-5663 → ACNP
Volume
16
Issue
9-12
Year of publication
2001
Pages
1119 - 1126
Database
ISI
SICI code
0956-5663(200112)16:9-12<1119:DOAQRB>2.0.ZU;2-L
Abstract
Theoretical and practical insights into the design and development of immob ilised enzyme inhibition biosensors are reported. A general mathematical ex pression relating the percent of enzyme inhibition (i.e. the analytical sig nal) to both the inhibitor concentration and the incubation time is present ed. The relevant physical, chemical and biochemical parameters required by the model are developed and discussed in terms of the inhibition of acetylc holinesterase by the organophosphorous pesticide, paraoxon. A second enzyme , choline oxidase and an amperometric transducer are used to facilitate the determination acetylcholinesterase inhibitor. (C) 2001 Elsevier Science B. V. All rights reserved.