Investigating the kinetics of DNA-DNA and PNA-DNA interactions using surface plasmon resonance-enhanced fluorescence spectroscopy

Citation
D. Kambhampati et al., Investigating the kinetics of DNA-DNA and PNA-DNA interactions using surface plasmon resonance-enhanced fluorescence spectroscopy, BIOSENS BIO, 16(9-12), 2001, pp. 1109-1118
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology
Journal title
BIOSENSORS & BIOELECTRONICS
ISSN journal
0956-5663 → ACNP
Volume
16
Issue
9-12
Year of publication
2001
Pages
1109 - 1118
Database
ISI
SICI code
0956-5663(200112)16:9-12<1109:ITKODA>2.0.ZU;2-H
Abstract
Plasmon surface polaritons, resonantly excited in the Kretschmann format, a re used to enhance the fluorescence emission of chromophore-labeled oligonu cleotides (15mers) binding to surface-attached (via biotin-streptavidin lin kages) complement catcher probes. A detailed analysis of the association an d dissociation kinetics as well as the affinity constants is given for a mi smatch I hybrid, emphasizing, in particular, the experimental conditions th at are required to allow for an artifact-free determination of rate constan ts. A first comparison between DNA- and peptide nucleic acid (PNA-) probes shows similar affinities, however, significant deviations from single-expon ential kinetics predicted by a simple Langmuir model for the PNA case are f ound. (C) 2001 Elsevier Science B.V. All rights reserved.