Induction of apoptosis in human gastric cancer cell lines by the polyaminesynthesis inhibitor, methylglyoxal bis(cyclopentylamidinohydrazone) (MGBCP)

Citation
S. Nakashima et al., Induction of apoptosis in human gastric cancer cell lines by the polyaminesynthesis inhibitor, methylglyoxal bis(cyclopentylamidinohydrazone) (MGBCP), BIOG AMINE, 16(4-5), 2001, pp. 327-342
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOGENIC AMINES
ISSN journal
0168-8561 → ACNP
Volume
16
Issue
4-5
Year of publication
2001
Pages
327 - 342
Database
ISI
SICI code
0168-8561(2001)16:4-5<327:IOAIHG>2.0.ZU;2-P
Abstract
Polyamines are important intracellular mediators of cell proliferation in c ancer cells. In this study, we evaluated whether methylglyoxal bis(cyclopen tylamidinohydrazone) (MGBCP), a polyamine synthesis inhibitor, induces apop tosis and inhibits growth in gastric cancer cell lines (MKN-1, MKN-28, MKN- 45). For comparison, the same experiment was carried out in a normal rat ga stric epithelial cell line (RGM-1). The growth of gastric cancer cell lines was dose-dependently inhibited by MGBCP. Almost all the cells became apopt otic when they were cultured in the presence of 40 muM MGBCP for 5 days. Ve ry high concentration (200 muM) of MGBCP was needed to completely inhibit t he proliferation of RGM-1 cells. The cellular concentrations of the polyami nes,spermidine and spermine were significantly reduced (50%) when the gastr ic cancer cells were cultured in the presence of MGBCP (80 muM) for 5 days. Putrescine remained unchanged. The reductions of both spermidine and sperm ine were mild in RGM-1 cells. DNA fragmentation and TUNEL studies demonstra ted the occurrence of apoptosis in gastric cancer cell lines but not in RGM -1 cells. The results of this study suggest the potential usefulness of MGB CP as an anti-cancer agent in gastric cancer.