Expression of SR-PSOX, a novel cell-surface scavenger receptor for phosphatidylserine and oxidized LDL in human atherosclerotic lesions

Citation
M. Minami et al., Expression of SR-PSOX, a novel cell-surface scavenger receptor for phosphatidylserine and oxidized LDL in human atherosclerotic lesions, ART THROM V, 21(11), 2001, pp. 1796-1800
Citations number
37
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Cardiovascular & Hematology Research
Journal title
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
ISSN journal
1079-5642 → ACNP
Volume
21
Issue
11
Year of publication
2001
Pages
1796 - 1800
Database
ISI
SICI code
1079-5642(200111)21:11<1796:EOSANC>2.0.ZU;2-N
Abstract
Receptor-mediated endocytosis of oxidized low density lipoprotein (Ox-LDL) by macrophages and the subsequent foam cell transformation in the arterial intima are key events in early atherogenesis. Recently, we have identified a novel macrophage cell-surface receptor for Ox-LDL by expression cloning f rom a cDNA library of phorbol 12-myristate 13-acetate-stimulated THP-1 cell s, designated as the scavenger receptor for phosphatidylserine and oxidized lipoprotein (SR-PSOX). Here, we examined SR-PSOX expression in human ather osclerotic lesions. Total cellular RNA and fresh frozen sections were prepa red from human carotid endarterectomy specimens (from 21 patients) and dire ctional coronary atherectomy specimens (from 11 patients). Fragments of hum an aortas of 2 patients without visible atherosclerotic lesions served as n egative controls. Quantitative reverse transcription-polymerase chain react ion demonstrated that SR-PSOX mRNA expression was prominent in atherosclero tic lesions but undetectable in normal aortas. Immunohistochemistry showed that SR-PSOX was predominantly expressed by lipid-laden macrophages in the intima of atherosclerotic plaques in carotid endarterectomy and directional coronary atherectomy specimens, although its expression was not detectable in normal arterial wall. Double-labeled immunohistochemistry confirmed tha t SR-PSOX is expressed by intimal macrophages. Taken together; SR-PSOX may be involved in Ox-LDL uptake and subsequent foam cell transformation in mac rophages in vivo and thus may play important roles in human atherosclerotic lesion formation.