Characterization of full-length HIV type 1 subtype C sequences from South Africa

Citation
J. Van Harmelen et al., Characterization of full-length HIV type 1 subtype C sequences from South Africa, AIDS RES H, 17(16), 2001, pp. 1527-1531
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AIDS RESEARCH AND HUMAN RETROVIRUSES
ISSN journal
0889-2229 → ACNP
Volume
17
Issue
16
Year of publication
2001
Pages
1527 - 1531
Database
ISI
SICI code
0889-2229(200111)17:16<1527:COFHT1>2.0.ZU;2-#
Abstract
Four full-length genome subtype C sequences from South Africa, three of whi ch are being used for vaccine development, were characterized. Three isolat es were obtained from recently infected individuals in KwaZulu/Natal: Du151 , Du422, and Du179. A fourth isolate, CTSc2, was obtained from an individua l residing in Cape Town. All four strains used the CCR5 coreceptor, althoug h Du179 also used CXCR4. The four isolates clustered within subtype C, but the three Du isolates formed a subcluster with a bootstrap value of 100%, w ith CTSc2 outside the subcluster. None of the strains showed evidence of in tersubtype recombination, as expected from the predominance of subtype C in South Africa. All 4 isolates had a 16-amino acid truncation on the 3' end of the Rev protein, identified in other subtype C isolates. Like many other subtype C strains, Du151, Du422, and Du179 had three NF-kappaB-binding sit es in the LTR; however, CTSc2 had only two.