No evidence for chromosomal instability in radiation workers with in vivo exposure to plutonium

Citation
Ca. Whitehouse et Ej. Tawn, No evidence for chromosomal instability in radiation workers with in vivo exposure to plutonium, RADIAT RES, 156(5), 2001, pp. 467-475
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
RADIATION RESEARCH
ISSN journal
0033-7587 → ACNP
Volume
156
Issue
5
Year of publication
2001
Part
1
Pages
467 - 475
Database
ISI
SICI code
0033-7587(200111)156:5<467:NEFCII>2.0.ZU;2-8
Abstract
The availability of cultured lymphocyte preparations from radiation workers with internal deposits of plutonium provided the opportunity to examine wh ether irradiation of bone marrow cells had induced a transmissible genomic. instability manifesting as an increase in de novo chromosome aberrations i n descendant cells in the peripheral blood. The men were originally classif ied as having more than 20% of the maximum permissible body burdens of plut onium, and recent red bone marrow dose calculations provided individual cum ulative estimates at the time of sampling ranging up to 1.8 Sv. The initial sampling occurred approximately 10 years after the main major intake, and samples were subsequently taken during three further periods over the follo wing 20 years. Control samples were available from three of the four sampli ng times. Chromosome analysis of solid Giemsa-stained material revealed no significant differences either in comparisons between the total group of pl utonium workers and controls for comparable periods or when the comparisons were restricted to a group of plutonium workers with initial red bone marr ow plutonium doses greater than 0.25 Sv. However, the frequencies of cells containing chromatid exchanges, chromatid breaks, and chromosome and chroma tid gaps decreased significantly over the study period for both the plutoni um workers as a whole and the controls, and a similar fluctuating pattern w as seen when sequential samples from groups of the same individuals were ex amined. Cells with dicentrics, centric rings and excess acentric fragments remained at similar frequencies throughout the study period. There was ther efore no evidence from the study of blood lymphocytes for the induction of persistent transmissible genomic. instability in the bone marrow of radiati on workers with internal deposits of plutonium. The work has, however, conf irmed the need for appropriate controls when conducting studies of cytogene tic end points of instability. (C) 2001 by Radiation Research Society.