Response of base excision repair enzymes to complex DNA lesions

Citation
M. Weinfeld et al., Response of base excision repair enzymes to complex DNA lesions, RADIAT RES, 156(5), 2001, pp. 584-589
Citations number
26
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Experimental Biology
Journal title
RADIATION RESEARCH
ISSN journal
0033-7587 → ACNP
Volume
156
Issue
5
Year of publication
2001
Part
2
Pages
584 - 589
Database
ISI
SICI code
0033-7587(200111)156:5<584:ROBERE>2.0.ZU;2-8
Abstract
There is now increasing evidence that ionizing radiation generates complex DNA damage, i.e. two or more lesions-single-strand breaks or modified nucle osides-located within one to two helical turns on the same strand or on opp osite strands. Double-strand breaks are the most readily recognizable clust ered lesions, but they may constitute a relatively minor fraction of the to tal. It is anticipated that clustered lesions may play a significant role i n cellular response to ionizing radiation since they may present a major ch allenge to the DNA repair machinery. The degree of lesion complexity increa ses with increasing LET. This has potential implications for space travel b ecause of exposure to high-LET cosmic radiation. It is therefore critical t hat we begin to understand the consequences of such damaged sites, includin g their influence on DNA repair enzymes. This paper presents a short review of our current knowledge of the action of purified DNA repair enzymes belo nging to the base excision repair pathway, including DNA glycosylases and a purinic/apyrimidinic endonucleases, on model complex lesions. (C) 2001 by R adiation Research Society.