Physiological concentrations of genistein stimulate the proliferation and protect against free radical-induced oxidative damage of MC3T3-E1 osteoblast-like cells

Citation
Ys. Lee et al., Physiological concentrations of genistein stimulate the proliferation and protect against free radical-induced oxidative damage of MC3T3-E1 osteoblast-like cells, NUTR RES, 21(9), 2001, pp. 1287-1298
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Endocrinology, Nutrition & Metabolism
Journal title
NUTRITION RESEARCH
ISSN journal
0271-5317 → ACNP
Volume
21
Issue
9
Year of publication
2001
Pages
1287 - 1298
Database
ISI
SICI code
0271-5317(200109)21:9<1287:PCOGST>2.0.ZU;2-4
Abstract
Genistein, a phytoestrogen and an antioxidant, acts on osteoblast-like cell s by increasing cellular proliferation. The present, study was designed to determine the antioxidative action of genistein on the viability of MC3T3-E 1 cells under oxidative stress induced by free radicals during cell prolife ration. After incubation for 24 hr, cells were treated with oxidative catal ysts, H2O2 and FeSO4, and the isoflavones (genistein and daidzein) for anot her 6 hr. Also, cells were treated with genistein or antioxidants (vitamin E or C) with or without H2O2 and FeSO4. The viability of cells treated with free radicals was significantly decreased (p < 0.05), whereas treatment wi th genistein at physiological concentrations reduced the damage to cells by free radicals over 6 hr (p < 0.05), and was more effective than either vit amin E or C. These results suggest that genistein not only stimulates cell proliferation but also protects against oxidative cellular damage of free r adicals in osteoblast-like cells. (C) 2001 Elsevier Science Inc. All rights reserved.