The 14-3-3 cerebrospinal fluid immunoassay lacks utility in the diagnosis of clinical scrapie

Citation
Jw. Tyler et al., The 14-3-3 cerebrospinal fluid immunoassay lacks utility in the diagnosis of clinical scrapie, J VET D INV, 13(6), 2001, pp. 537-539
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Veterinary Medicine/Animal Health
Journal title
JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION
ISSN journal
1040-6387 → ACNP
Volume
13
Issue
6
Year of publication
2001
Pages
537 - 539
Database
ISI
SICI code
1040-6387(200111)13:6<537:T1CFIL>2.0.ZU;2-I
Abstract
This study determined whether the immunoassay for cerebrospinal fluid 14-3- 3 protein concentration was sensitive and specific in the diagnosis of natu rally occurring clinical scrapie. in sheep. Cerebrospinal fluid was collect ed from 9 sheep with the confirmed diagnosis of scrapie. Additionally, cere brospinal fluid was collected from 13 clinically normal sheep, which origin ated from a closely monitored flock with no history of scrapie. Sensitivity and specificity were calculated using standard epidemiological methods. Ce rebrospinal fluid immunoassay results did not differ significantly between positive and negative sheep. Test sensitivity varied from 0.55 to 0.66, dep ending on the choice of test endpoint. Test specificity varied from 0.30 to 0.77, depending on the choice of test endpoint. The 14-3-3 cerebrospinal f luid immunoassay appears to have no value in the diagnosis of clinical scra pie in sheep.