The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium

Citation
Yq. Huo et al., The chemokine KC, but not monocyte chemoattractant protein-1, triggers monocyte arrest on early atherosclerotic endothelium, J CLIN INV, 108(9), 2001, pp. 1307-1314
Citations number
48
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research General Topics
Journal title
JOURNAL OF CLINICAL INVESTIGATION
ISSN journal
0021-9738 → ACNP
Volume
108
Issue
9
Year of publication
2001
Pages
1307 - 1314
Database
ISI
SICI code
0021-9738(200111)108:9<1307:TCKBNM>2.0.ZU;2-I
Abstract
In a reconstituted flow chamber system, preincubation with chemokines can t rigger the arrest of rolling monocytes, suggesting that this interaction co uld help recruit these cells to early atherosclerotic lesions. To date, how ever, the contribution of endothelium-derived chemokines found in these les ion to monocyte arrests has not been investigated. The endothelium of lesio n-prone carotid arteries from apolipoprotein E-deficient (ApoE(-/-)) mice, but not control mice, presents the chemokines KC (mouse GRO-alpha) and JE ( mouse monocyte chemoattractant protein-1 [MCP-1]). Arrest of a monocytic ce ll line or mouse blood monocytes perfused through carotid arteries of ApoE( -/-) mice was reduced by treating with either pertussis toxin, an antagonis t of CXCR2, or an antibody to KC, but this process was insensitive to agent s that blocked CCR-2 or JE. Conversely, monocyte accumulation more than dou bled upon pre-perfusion of the carotid artery with KC but not with mouse MC P-1. Blockade Of alpha (4)beta (1) integrin (VLA-4) or vascular cell adhesi on molecule-1, but not CD18 or intercellular adhesion molecule-1, almost co mpletely inhibited the arrest of monocytes. We conclude that when presented by early atherosclerotic lesions, KC but not murine MCP-1 triggers VLA-4-d ependent monocyte recruitment.