Production of L-erythrose via L-erythrulose from erythritol using microbial and enzymatic reactions

Citation
Rm. Mizanur et al., Production of L-erythrose via L-erythrulose from erythritol using microbial and enzymatic reactions, J BIOSCI BI, 92(3), 2001, pp. 237-241
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
92
Issue
3
Year of publication
2001
Pages
237 - 241
Database
ISI
SICI code
1389-1723(200109)92:3<237:POLVLF>2.0.ZU;2-C
Abstract
A rare aldotetrose, L-erythrose, was produced from erythritol via a two-ste p reaction. In the first step, complete oxidation of erythritol to L-erythr ulose was achieved by using Gluconobacter frateurii IFO 3254. Washed cell s uspension of the strain grown on tryptic soy broth (TSB) supplemented with 1% D-sorbitol was used to carry out the transformation reaction at 30 degre esC with shaking at 170 rpm. At 10% substrate concentration, 98% erythritol was converted to L-erythrulose within 48 h. The produced L-erythrulose was then used as a substrate for the production of L-erythrose. The isomerizat ion of L-erythrulose to L-erythrose was carried out using constitutively pr oduced L-ribose isomerase (L-RI) from the mutant strain Acinetobacter sp. D L-28 grown on D-lyxose mineral salt medium. At equilibrium, the yield of L- erythrose from L-erythrulose was 18% and finally 1.7 g L-erythrose was obta ined from 10 g erythritol. After a number of simple purification steps, the product was isolated from the reaction mixture by ion-exchange column chro matography (Dowex 50W-X2, Ca2+). The structure of the product was determine d after NaBH4 reduction from Infrared (IR) and C-13 nuclear magnetic resona nce (NMR) spectra.