Optimal conditions for production of (R)-1-Phenylpropanol by Fusarium moniliforme strain MS31

Citation
A. Uzura et al., Optimal conditions for production of (R)-1-Phenylpropanol by Fusarium moniliforme strain MS31, J BIOSCI BI, 92(3), 2001, pp. 288-293
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biotecnology & Applied Microbiology",Microbiology
Journal title
JOURNAL OF BIOSCIENCE AND BIOENGINEERING
ISSN journal
1389-1723 → ACNP
Volume
92
Issue
3
Year of publication
2001
Pages
288 - 293
Database
ISI
SICI code
1389-1723(200109)92:3<288:OCFPO(>2.0.ZU;2-S
Abstract
Resting cells of Fusarium moniliforme strain MS31 produced (R)-1-phenylprop anol front propylbenzene. The components of the medium and the reaction con ditions were adjusted to increase the specific activity of the hydroxylatin g enzyme involved. Glucose and sodium nitrate were selected as carbon and n itrogen sources, respectively. The substrate, propylbenzene, inhibited fung al growth and the activity of the enzyme. Acetoin added to the medium incre ased both growth and activity of the enzyme, and hydroxylation of propylben zene increased by 1.4-fold. Maximum bioconversion of propylbenzene by resti ng cells of the fungus was at 25-30 degreesC and pH 7.0 with cells at conce ntration of 40 mg (dry) per milliliter of reaction mixture. Conversion was accelerated as soon as propylbenzene was added; slowing 2 h later. In the e nd, F. moniliforme strain MS31 produced (R)-1-phenylpropanol with an enanti omeric excess of 98% at the concentration of 16 mM (2.2 mg . ml(-1)).