The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with Dengue virus core protein

Citation
Cj. Chang et al., The heterogeneous nuclear ribonucleoprotein K (hnRNP K) interacts with Dengue virus core protein, DNA CELL B, 20(9), 2001, pp. 569-577
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
DNA AND CELL BIOLOGY
ISSN journal
1044-5498 → ACNP
Volume
20
Issue
9
Year of publication
2001
Pages
569 - 577
Database
ISI
SICI code
1044-5498(200109)20:9<569:THNRK(>2.0.ZU;2-A
Abstract
Heterogeneous nuclear ribonucleoprotein K (hnRNP K), a component of hnRNP p articles, is involved in several steps of gene expression regulation. Dengu e (DEN) virus, a member of the Flaviviridae, is the primary cause of illnes ses such as dengue fever, dengue hemorrhagic fever, and dengue shock syndro me. In mature DEN virus particles, the core protein is a structural protein that forms a nucleocapsid complex with genomic RNA. Very little of its bio logic functions is known. Here, using an in vitro binding assay and coimmun oprecipitation analysis, we report a protein-protein interaction between th e DEN virus core protein and hnRNP K. The C-terminal hydrophilic region of the DEN virus core protein, spanning amino acid residues 73 to 100, is requ ired for such interaction. Results of glutathione-S transferase binding ass ays indicated that the core protein-hnRNP K interaction might be abolished in the presence of hnRNP K cognate nucleic acids. Furthermore, in a cotrans fection experiment, the repressive effect of hnRNP K on C/EBP beta -mediate d transcription activation could be reversed by full-length DEN virus core protein but not by a truncated form containing amino acids 1-72. Our result s suggest that, on DEN virus infection, the multiple functions of cellular hnRNP K may be affected by the virus core protein.