Mevalonate pyrophosphate decarboxylase is predominantly located in the cytosol of rat hepatocytes

Citation
A. Michihara et al., Mevalonate pyrophosphate decarboxylase is predominantly located in the cytosol of rat hepatocytes, BIOL PHAR B, 24(11), 2001, pp. 1235-1240
Citations number
23
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
0918-6158 → ACNP
Volume
24
Issue
11
Year of publication
2001
Pages
1235 - 1240
Database
ISI
SICI code
0918-6158(200111)24:11<1235:MPDIPL>2.0.ZU;2-8
Abstract
Mevalonate pyrophosphate decarboxylase (MPD) is found in the 100000xg super natant fraction of cells or tissues and is considered to be a cytosolic pro tein. Recently, other groups reported that MPD is mostly located in the per oxisomes. In this study, we used two different methods to determine whether MPD is predominantly located in the peroxisomes or the cytosol of rat hepa tocytes. 1) In permeabilized rat hepatocytes or normal rat kidney cells tre ated with digitonin, which lack cytosolic enzyme, MPD was mainly present in the medium. 2) Double immunofluorescent labeling of cells with both anti-M PD antibody and anti-hexokinase antibody yielded an immunofluorescent patte rn for both enzymes typical of the cytosolic protein. These results indicat e that MPD is predominantly located in the cytosol of rat hepatocytes.