Tissue distribution of a major mevalonate pyrophosphate decarboxylase in rats

Citation
A. Michihara et al., Tissue distribution of a major mevalonate pyrophosphate decarboxylase in rats, BIOL PHAR B, 24(11), 2001, pp. 1231-1234
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Pharmacology & Toxicology
Journal title
BIOLOGICAL & PHARMACEUTICAL BULLETIN
ISSN journal
0918-6158 → ACNP
Volume
24
Issue
11
Year of publication
2001
Pages
1231 - 1234
Database
ISI
SICI code
0918-6158(200111)24:11<1231:TDOAMM>2.0.ZU;2-H
Abstract
The 45- and 35-kDa subunits of mevalonate pyrophosphate decarboxylase (MPD) have been purified from rat liver. In this study, we examined the relation ship between 45- and 35-kDa MPD and the tissue distribution of a major MPD in rat liver. When the crude extract of rat liver fed on normal chow was su bjected to immunoblot analysis using anti-rat 45-kDa MPD antibody, only the 45-kDa band was detected. In a pulse-chase experiment using anti-rat 45-kD a MPD antibody, there was no precursor-product relationship between the 45- and the 35-kDa MPD. In immunoprecipitation, more than 85% of MPD activity in the rat liver was depleted from the crude extract with an excess of the above antibody. When 45-kDa MPD contents in tissues were analyzed by immuno blotting, a single protein band with an apparent molecular weight of 45 kDa was detected in all tissues. The specific protein content of 45-kDa MPD in liver was markedly higher than in other tissues. The activity/amount ratio varied among brain, liver, and testis, being significantly highest in the liver. From these data, it is suggested that 45-kDa MPD serves as a major e nzyme involved in cholesterol biosynthesis in rat liver and that a tissue-s pecific regulator or isozyme of 45-kDa MPD is present in rat liver.