EFFECTS OF SALINOMYCIN AND VITAMIN-B-6 ON THE IN-VITRO SYNTHESIS OF LYSINE FROM THE STEREOISOMERS OF 2,6-DIAMINOPIMELIC ACID BY MIXED RUMENPROTOZOA, BACTERIA, AND THEIR MIXTURE

Citation
Am. Elwaziry et R. Onodera, EFFECTS OF SALINOMYCIN AND VITAMIN-B-6 ON THE IN-VITRO SYNTHESIS OF LYSINE FROM THE STEREOISOMERS OF 2,6-DIAMINOPIMELIC ACID BY MIXED RUMENPROTOZOA, BACTERIA, AND THEIR MIXTURE, Journal of General and Applied Microbiology, 43(2), 1997, pp. 109-114
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology,"Biothechnology & Applied Migrobiology
ISSN journal
0022-1260
Volume
43
Issue
2
Year of publication
1997
Pages
109 - 114
Database
ISI
SICI code
0022-1260(1997)43:2<109:EOSAVO>2.0.ZU;2-Z
Abstract
An in vitro study was conducted to examine the effects of salinomycin (SL) and vitamin B-6 (pyridoxine hydrochloride) (B-6) on the productio n of lysine from the three stereoisomers of 2,6-diaminopimelic acid (D AP-SI) by mixed rumen protozoa (P), mixed rumen bacteria (a), and thei r mixture (PB), P, a, and PB were isolated from the rumen of goats giv en a concentrate mixture and lucerne cubes, separately incubated for 1 2 h with and without DAP-SI (5 mM) as a substrate and SL (5 mu g/ml) a nd/or B-6 (10 mu g/ml) as additives, In P suspensions, SL and B-6 redu ced the amount of DAP-SI by 2.1 times (p<0.001, where p is probability ) and 19.9% (p<0.05), respectively, and also increased the production of lysine by 2.4 times (p<0.001) and 26.8% (p<0.05), respectively, dur ing 12 h incubation, In a suspensions, the reductions of DAP-SI with a single addition of SL or B-6 were 8.5% (p<0.001) and 2.7%, respective ly, and lysine production increased by 54.3 and 32.9% (p<0.001), respe ctively, during 12h incubation, In PB suspensions, the reductions of D AP-SI were 21.9 and 11.7% (p<0.001) with a single addition of SL or B- 6, respectively, and the production of lysine increased by 81.4 and 39 .4% (p<0.001), respectively, during 12h incubation. When SL and B-6 we re added together to the P, a, and PB suspensions, lysine production f urther increased by 12.3, 21.3, and 12.4% more than the cases of addin g SL only during 12 h incubation, respectively, st, and B-6 were demon strated to enhance the production of lysine from DAP-SI by mixed rumen protozoa, mixed rumen bacteria and their mixture in this study.