Expression of skeletal muscle cells apoptosis regulatory proteins in plasma-perfused VX2 carcinoma-bearing rabbits

Citation
O. Ishiko et al., Expression of skeletal muscle cells apoptosis regulatory proteins in plasma-perfused VX2 carcinoma-bearing rabbits, ANTICANC R, 21(4A), 2001, pp. 2363-2368
Citations number
22
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
ANTICANCER RESEARCH
ISSN journal
0250-7005 → ACNP
Volume
21
Issue
4A
Year of publication
2001
Pages
2363 - 2368
Database
ISI
SICI code
0250-7005(200107/08)21:4A<2363:EOSMCA>2.0.ZU;2-T
Abstract
Background: In tumor-bearing animals we found that the skeletal muscle apop tosis might be involved in muscle wasting. In this study, we investigated c hanges in the skeletal muscle cell apoptosis regulatory proteins after cycl ic plasma-perfusion (CPP). Materials and Methods: bile studied changes in b ody weight, lean body mass (LBM), apoptotic index (AI) and expression of Ba x and Bcl-2 in skeletal muscle in VX2 carcinoma-bearing rabbits. Results: 2 0 days after tumor implantation, LBM had decreased by 5.06 +/- 1.10%, while the AI had increased to 40.5 +/- 3.20%. By 40 days, LBM had decreased by 1 1.0 +/- 0.81% and the AI was only 0.93 +/- 0.96%. Bax expression was detect ed in proportion to the AI, but no Bcl-2 expression was detected in either the experimental or control groups. CPP improved LBM, but did not prevent B ax expression. Conclusion: Skeletal muscle cell apoptosis related to Bax wa s concluded to be the cause of muscle wasting in VX2 carcinoma-bearing rabb its. CPP appears to reduce muscle wasting and increase LBM, but it did not suppress Bax expression or skeletal muscle cell apoptosis.