Enhancement of in vitro hsp72 expression by placental IL-6

Citation
S. Miranda et al., Enhancement of in vitro hsp72 expression by placental IL-6, AM J REPROD, 46(5), 2001, pp. 358-365
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
46
Issue
5
Year of publication
2001
Pages
358 - 365
Database
ISI
SICI code
1046-7408(200111)46:5<358:EOIVHE>2.0.ZU;2-X
Abstract
PROBLEM: To give an approach in order to elucidate the mechanism by which p lacental IL-6 induce modifications in the glycosylation status of immunoglo bulins, in the present work, we investigate a putative relationship between a stimulus by placental IL-6 and expression of cytoplasmic "hsp70 family p roteins" in an in vitro model. METHODS OF STUDY: Supernatants of cultures of placentae obtained from primi parous and multiparous AKR/J x AKR/J and AKR/J x BALB/c mouse crossbreeding s were added to mouse IgG1 hybridoma cultures which produced symmetric and asymmetric anti-dinitrophenol (anti-DNP) antibodies. Analyses of the expres sion of inducible hsp72/constitutive hsp73 in cellular lysates obtained fro m hybridomas cultured, in the presence of rmIL-6 or crude murine placental culture supernatants; followed by neutralization assays with anti IL-6, wer e performed. In addition, the level of IL-6 present in the employed placent al culture supernatants was determined and compared with the placental hsp7 0-inducing effect. RESULTS: These experiments showed that mouse placentae were able to release IL-6 in vitro. In addition, mouse placental supernatants (PS) containing o ver 1,000 pg/mL of IL-6 enhanced the expression of the inducible isoform hs p72 in the employed hybridomas. This effect was abolished when the hsp70-in ducing PS were previously incubated with anti-mIL6 antibody. CONCLUSIONS: These observations indicate that mouse placentae produce diffe rent titers of IL-6 and suggest that IL-6 appears to be the unique mouse pl acental factor able to induce in vitro hsp72 synthesis. A relationship with the increased synthesis of anti-paternal antigen asymmetric antibodies, pr eviously observed during pregnancy, is discussed.