Expression of interleukin-18 and its receptor in mouse ovary

Citation
Y. Tsuji et al., Expression of interleukin-18 and its receptor in mouse ovary, AM J REPROD, 46(5), 2001, pp. 349-357
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
46
Issue
5
Year of publication
2001
Pages
349 - 357
Database
ISI
SICI code
1046-7408(200111)46:5<349:EOIAIR>2.0.ZU;2-L
Abstract
PROBLEM: Interleukin-18 (IL-18) strongly induces interferon-gamma productio n and is produced not only by types of immune cells but also by types of no n-immune cells. Ovulation is thought to be an inflammation-like reaction in which many pro-inflammatory cytokines are involved. We investigated whethe r IL-18 is involved in the functions of ovary. METHOD OF STUDY: The 4-week-old immature female mice were examined for IL-1 8 and IL-18 receptor (IL-18R) expression on their ovaries under stimulation with pregnant mare's serum gonadotropin (PMSG) and human chorionic gonadot ropin (hCG) by immunohistochemical staining, Western blotting and reverse t ranscript-polymerase chain reaction. The IL-18R was blocked by the injectio n of anti-IL-18R monoclonal antibody to immature mice during PMSG-hCG stimu lation, and the number of ovulated ova was counted. RESULTS: The expression of both proteins and mRNA of IL-18 and IL-18R were very low in immature ovaries before stimulation, but after PMSG injection b oth IL-18 and IL-18R increased dramatically in theca cells and reached a ma ximum level at the peri-ovulatory phase then slightly lowered, but still ke pt a high level during the luteal phase in the corpus luteum. The treatment of IL-18R monoclonal antibody to the mice during ovarian stimulation reduc ed the number of ovulated ova and inhibited the expansion of cumulus cells surrounding the ovum. CONCLUSION: IL-18 and IL-18R play roles in various kinds of function of ova ry.