Expression of beta chemokines in explants and trophoblasts from early and term human placentae

Citation
M. Moussa et al., Expression of beta chemokines in explants and trophoblasts from early and term human placentae, AM J REPROD, 46(5), 2001, pp. 309-317
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Immunology
Journal title
AMERICAN JOURNAL OF REPRODUCTIVE IMMUNOLOGY
ISSN journal
1046-7408 → ACNP
Volume
46
Issue
5
Year of publication
2001
Pages
309 - 317
Database
ISI
SICI code
1046-7408(200111)46:5<309:EOBCIE>2.0.ZU;2-#
Abstract
PROBLEM: Implantation of human embryo requires expression of inflammatory c ytokines and local attraction of T cells and natural killer (NK) cells. Che mokines are chemoattractants for these cells in classical inflammation. We speculated that they could also be involved in implantation. METHOD OF STUDY: We assessed by enzyme-linked immunosorbent assay (ELISA), reverse transcriptase-polymerase chain reaction (RT-PCR) and immunohistoche mistry the presence of three classical beta chemokines Macrophage Inflammat ory Protein 1 (MIP1)alpha, MIP1 beta and Regulated upon activation, normal T cells expressed and secreted (RANTES) in cultures of placental villi or i solated trophoblasts derived from human first trimester and term placenta. RESULTS: Explant culture assays were positive for these three chemokines, w ith important quantitative variations between individuals. Half of the high ly purified trophoblasts cultures were found by ELISA and RT-PCR to secrete in vitro MIP1 alpha and MIP1 beta. RANTES was never detected by ELISA in t rophoblasts cultures, albeit we could detect a low amount of messenger RNA. Immunohistochemistry experiments show that Hofbauer cells and the trophobl ast layer are a secretion site of MIP1 beta in term placenta, and that cyto trophoblasts are able to secrete this chemokine in early placenta. CONCLUSION: Human placenta is a site of secretion of chemokines that could be involved in establishment of pregnancy.