An experimental study on the fluorescence correlation spectroscopy in laser gradient field

Authors
Ding, Y Meng, FB Chen, B Ma, H Lei, J Chen, DY
Citation
Y. Ding et al., An experimental study on the fluorescence correlation spectroscopy in laser gradient field, ACT PHY C E, 50(11), 2001, pp. 2269-2274
Citations number
13
Language
CHINESE
art.tipo
Article
Categorie Soggetti
Physics
Journal title
ACTA PHYSICA SINICA
ISSN journal
1000-3290 → ACNP
Volume
50
Issue
11
Year of publication
2001
Pages
2269 - 2274
Database
ISI
SICI code
1000-3290(200111)50:11<2269:AESOTF>2.0.ZU;2-V
Abstract
This work studies experimentally the influence of a laser gradient field on fluorescence correlation spectroscopy measurements. Results show that the derived diffusion time for particles across the detection volume increases almost linearly with the strength of the, gradient field. The derived numbe r of fluorescing particles in the detection volume also increases with the field. The larger the polarizability of the particles, the bigger the effec ts of the gradient field.