Quantification of TECTA and DFNA5 expression in the developing mouse cochlea

Citation
Y. Maeda et al., Quantification of TECTA and DFNA5 expression in the developing mouse cochlea, NEUROREPORT, 12(15), 2001, pp. 3223-3226
Citations number
15
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Neurosciences & Behavoir
Journal title
NEUROREPORT
ISSN journal
0959-4965 → ACNP
Volume
12
Issue
15
Year of publication
2001
Pages
3223 - 3226
Database
ISI
SICI code
0959-4965(20011029)12:15<3223:QOTADE>2.0.ZU;2-5
Abstract
TECTA and DFNA5 are the mouse orthologues of the human deafness-associated genes TECTA and DFNA5. To determine how expression of these genes is regula ted during development, relative mRNA abundance was examined in mice by non radioactive RT-PCR. TECTA mRNA was detected on embryonic day 15 (E15), incr eased to its highest level on postnatal day 3 (P3) and then dramatically de creased by P15. Low levels persisted (adulthood, P45 to 67) with mean mRNA abundance after P15 less than 25% of P3 levels. DFNA5 mRNA expression was c onstant throughout these time points. These results imply that TECTA is tra nscribed at a particularly high level during tectorial membrane morphogenes is. In contrast, DFNA5 is present in both the developing and mature cochlea . NeuroReport 12:3223-3226 (C) 2001 Lippincott Williams & Wilkins.