K. Furukawa et al., Effects of hormone and growth factor on formation of fibroblast-aggregatesfor tissue-engineered skin, MAT SCI E C, 17(1-2), 2001, pp. 59-62
To accelerate wound healing after implantation of tissue-engineered skin pr
oducts, higher density inoculation of fibroblasts would be important. There
fore, we attempted to develop a tissue-engineered skin with a higher densit
y of fibroblasts by formation of cell aggregates combined with a biodegrada
ble Polymer. Our method consisted of rotationally shaking with nontreated d
ishes, declining fibroblast-material interactions, and augmenting cell-cell
interaction. Under the culture condition, normal neonatal human dermal fib
roblasts formed spheroidal aggregates. The formation of the fibroblast-aggr
egates was accelerated by the addition of insulin, dexamethasone, basic fib
roblast growth factor, and ascorbic acid to basal medium, and by the contro
l of shaking time. In fibroblast-aggregates formed under a basal medium, th
e level of gene expression related to wound healing was higher than that of
a monolayer when one added the hormones and growth factors. Therefore, tis
sue-engineered skin products with aggregates and scaffolds would become an
artificial skin with potentiality of higher wound 'aealing. (C) 2001 Elsevi
er Science B.V. All rights reserved.