Effects of hormone and growth factor on formation of fibroblast-aggregatesfor tissue-engineered skin

Citation
K. Furukawa et al., Effects of hormone and growth factor on formation of fibroblast-aggregatesfor tissue-engineered skin, MAT SCI E C, 17(1-2), 2001, pp. 59-62
Citations number
10
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Apllied Physucs/Condensed Matter/Materiales Science
Journal title
MATERIALS SCIENCE & ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS
ISSN journal
0928-4931 → ACNP
Volume
17
Issue
1-2
Year of publication
2001
Pages
59 - 62
Database
ISI
SICI code
0928-4931(20011101)17:1-2<59:EOHAGF>2.0.ZU;2-2
Abstract
To accelerate wound healing after implantation of tissue-engineered skin pr oducts, higher density inoculation of fibroblasts would be important. There fore, we attempted to develop a tissue-engineered skin with a higher densit y of fibroblasts by formation of cell aggregates combined with a biodegrada ble Polymer. Our method consisted of rotationally shaking with nontreated d ishes, declining fibroblast-material interactions, and augmenting cell-cell interaction. Under the culture condition, normal neonatal human dermal fib roblasts formed spheroidal aggregates. The formation of the fibroblast-aggr egates was accelerated by the addition of insulin, dexamethasone, basic fib roblast growth factor, and ascorbic acid to basal medium, and by the contro l of shaking time. In fibroblast-aggregates formed under a basal medium, th e level of gene expression related to wound healing was higher than that of a monolayer when one added the hormones and growth factors. Therefore, tis sue-engineered skin products with aggregates and scaffolds would become an artificial skin with potentiality of higher wound 'aealing. (C) 2001 Elsevi er Science B.V. All rights reserved.