Purification and some properties of an inhibitor for a yeast pleiotropic drug resistant pump from Kitasatospora sp E-420

Citation
A. Wanigasekera et al., Purification and some properties of an inhibitor for a yeast pleiotropic drug resistant pump from Kitasatospora sp E-420, BIOS BIOT B, 65(10), 2001, pp. 2353-2357
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Agricultural Chemistry","Biochemistry & Biophysics
Journal title
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
ISSN journal
0916-8451 → ACNP
Volume
65
Issue
10
Year of publication
2001
Pages
2353 - 2357
Database
ISI
SICI code
0916-8451(200110)65:10<2353:PASPOA>2.0.ZU;2-0
Abstract
A strain producing an inhibitor for pleiotropic drug resistant 5 (Pdr5) was isolated using our original screening system in yeast. The strain was clas sified and named as Kitasatospora sp. E-420. The purified inhibitor (molecu lar weight = 1,193 by FAB-MS) inhibited a Pdr5-mediated efflux of cyclohexi mide and cerulenin. The intracellular accumulation of a fluorescent dye, rh odamine 123, by the inhibitor was also confirmed. Some physicochemical data suggested that the Pdr5-specific inhibitor was different from an immunosup pressant, FK506, reported as the only inhibitor of Pdr5 in vivo.