Identification of a homopolymer of 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-nonulosonic acid in the lipopolysaccharides of Legionella pneumophila non-1 serogroups

Citation
Ya. Knirel et al., Identification of a homopolymer of 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxy-D-glycero-D-talo-nonulosonic acid in the lipopolysaccharides of Legionella pneumophila non-1 serogroups, BIOCHEM-MOS, 66(9), 2001, pp. 1035-1041
Citations number
20
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biochemistry & Biophysics
Journal title
BIOCHEMISTRY-MOSCOW
ISSN journal
0006-2979 → ACNP
Volume
66
Issue
9
Year of publication
2001
Pages
1035 - 1041
Database
ISI
SICI code
0006-2979(200109)66:9<1035:IOAHO5>2.0.ZU;2-G
Abstract
O-Specific polysaccharides (OPS) were isolated by mild acid hydrolysis of t he lipopolysaccharides (LPS) of strains of Legionella pneumophila serogroup s 2-14, as well as strains Lansing 3 and 16453-92 from newly proposed serog roups. The OPS were Studied by H-1- and C-13-NMR spectroscopy, GLC/mass spe ctrometry and chemical modifications (mild alkaline O-deacetylation and con version of the N-acetimidoyl group into the N-acetyl group). All OPS were f ound to be a homopolymer of a 5-acetamidino-7-acetamido-3,5,7,9-tetradeoxyn onulosonic acid, which in some strains is 8-O-acetylated. In most strains S tudied, the monosaccharide has the D-glycero-D-talo configuration and is th us the C4 epimer of legion-aminic acid (4-epilegionaminic acid), which has been previously identified as the monomer in the OPS of L. pneumophila sero group 1. Poly(4-epilegionaminic acid) occurs as a minor polysaccharide in s erogroups 5 (strain Dallas 1) and 13 and is absent in serogroups 1 and 7. T he chemical basis for serological differentiation of L. pneumophila strains is discussed.