Two distinct monooxygenases for alkane oxidation in Nocardioides sp strainCF8

Citation
N. Hamamura et al., Two distinct monooxygenases for alkane oxidation in Nocardioides sp strainCF8, APPL ENVIR, 67(11), 2001, pp. 4992-4998
Citations number
50
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Biology,Microbiology
Journal title
APPLIED AND ENVIRONMENTAL MICROBIOLOGY
ISSN journal
0099-2240 → ACNP
Volume
67
Issue
11
Year of publication
2001
Pages
4992 - 4998
Database
ISI
SICI code
0099-2240(200111)67:11<4992:TDMFAO>2.0.ZU;2-Z
Abstract
Alkane monooxygenases in Nocardioides sp. strain CF8 were examined at the p hysiological and genetic levels. Strain CF8 can utilize alkanes ranging in chain length from C-2 to C-16. Butane degradation by butane-grown cells was strongly inhibited by allylthiourea, a copper-selective chelator, while he xane-, octane-, and decane-grown cells showed detectable butane degradation activity in the presence of allylthiourea. Growth on butane and hexane was strongly inhibited by 1-hexyne, while 1-hexyne did not affect growth on oc tane or decane. A specific 30-kDa acetylene-binding polypeptide was observe d for butane-, hexane-, octane-, and decane-grown cells but was absent from cells grown with octane or decane in the presence of 1-hexyne. These resul ts suggest the presence of two monooxygenases in strain CF8. Degenerate pri mers designed for PCR amplification of genes related to the binuclear-iron- containing alkane hydroxylase from Pseudomonas oleovorans were used to clon e a related gene from strain CF8. Reverse transcription-PCR and Northern bl ot analysis showed that this gene encoding a binuclear-iron-containing alka ne hydroxylase was expressed in cells grown on alkanes above C-6. These res ults indicate the presence of two distinct monooxygenases for alkane oxidat ion in Nocardioides sp. strain CF8.