Absence of somatic hypermutation of immunoglobulin heavy chain variable region genes in precursor B-lymphoblastic lymphoma - A study of four cases inchildhood and adolescence

Citation
H. Hojo et al., Absence of somatic hypermutation of immunoglobulin heavy chain variable region genes in precursor B-lymphoblastic lymphoma - A study of four cases inchildhood and adolescence, AM J CLIN P, 116(5), 2001, pp. 673-682
Citations number
38
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Research/Laboratory Medicine & Medical Tecnology","Medical Research Diagnosis & Treatment
Volume
116
Issue
5
Year of publication
2001
Pages
673 - 682
Database
ISI
SICI code
Abstract
Somatic mutation (SM) analysis provides a useful tool for understanding the suitable stage of differentiation of normal and neoplastic B cells. B-cell precursor neoplasms are considered to be somatically premutational. Howeve r; no reports have been made on the sequence analysis of SM in rearranged i mmunoglobulin heavy chain variable region (VH) genes in precursor B-lymphob lastic lymphoma (PB-LBL); as for B-cell acute lymphoblastic leukemia (B-ALL ), the variable frequency of SM of the VH genes has been described. To better distinguish PB-LBL from B-ALL based on the stage at which differe ntiation occurs, we analyzed the SM of the VH genes in 4 children and adole scents with PB-LBL. In all 4 cases, there was no SM in complementarity-dete rmining regions 1, 2, and 3 and in framework regions 2 and 3 of the VH gene s, except for 1 mutation in 1 of 2 polymerase chain reaction products from 1 patient. Our data showed the absence of SM of lymphoma cells in PB-LBL, i n addition to the developmentally regulated rearrangement of VH genes at th e stage of B-cell precursor cells. The lack of mutation in VH gene sequence s suggests that SM may differ between PB-LBL and B-ALL.