Establishment of gene-trap and enhancer-trap systems in the moss Physcomitrella patens

Hiwatashi, Y Nishiyama, T Fujita, T Hasebe, M

Y. Hiwatashi et al., Establishment of gene-trap and enhancer-trap systems in the moss Physcomitrella patens, PLANT J, 28(1), 2001, pp. 105-116

52

INGLESE

Article

Plant Sciences","Animal & Plant Sciences

PLANT JOURNAL

0960-7412 → ACNP

28

1

2001

105 - 116

ISI

0960-7412(200110)28:1<105:EOGAES>2.0.ZU;2-4

Because of its simple body plan and ease of gene knockout and allele replac ement, the moss Physcomitrella patens is often used as a model system for s tudies in plant physiology and developmental biology. Gene-trap and enhance r-trap systems are useful techniques for cloning genes and enhancers that f unction in specific tissues or cells. Additionally, these systems are conve nient for obtaining molecular markers specific for certain developmental pr ocesses. Elements for gene-trap and enhancer-trap systems were constructed using the uidA reporter gene with either a splice acceptor or a minimal pro moter. Through a high rate of transformation conferred by a method utilizin g homologous recombination, 235 gene-trap and 1073 enhancer-trap lines were obtained from 5637 and 3726 transgenic lines, respectively. The expression patterns of these trap lines in the moss gametophyte varied. The candidate gene trapped in a gene-trap line YH209, which shows rhizoid-specific expre ssion, was obtained by 5' and 3' RACE. This gene was named PpGLU, and forms a clade with plant acidic alpha -glucosidase genes. Thus, these gene-trap and enhancer-trap systems should prove useful to identify tissue- and cell- specific genes in Physcomitrella.