Ribotyping of entomopathogenic Verticillium lecanii in Japan

Citation
M. Sugimoto et al., Ribotyping of entomopathogenic Verticillium lecanii in Japan, PHYTOPARASI, 29(5), 2001, pp. 413-420
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Plant Sciences
Journal title
PHYTOPARASITICA
ISSN journal
0334-2123 → ACNP
Volume
29
Issue
5
Year of publication
2001
Pages
413 - 420
Database
ISI
SICI code
0334-2123(2001)29:5<413:ROEVLI>2.0.ZU;2-5
Abstract
To estimate the genetic diversity in 30 isolates of Verticillium lecanii fr om aphids, whiteflies, mite and black pine in Japan, including two commerci alized strains (Mycotal and Vertalec), DNA polymorphisms in ribosomal DNA o f those isolates were analyzed using polymerase chain reaction (PCR). The i nternal transcribed spacer (ITS) and intergenic spacer (IGS) regions of the nuclear ribosomal RNA gene of each isolate were analyzed by PCR-RFLP (rest riction fragment length polymorphism). The size of the PCR product from the ITS region was similar to 580 bp in 27 of the isolates. A 600 bp ITS produ ct was detected in Mycotal and Vertalec. One Japanese. isolate produced bot h the 580 bp and 600 bp products. Enzymatic digestion of the ITS region wit h Sau3A I, Msp I, Hae III and Rsa I revealed RFLPs that consisted of eight haplotypes. Mycotal and Vertalec were specific haplotypes that differed fro m other isolates. The Japanese isolates had a complex relationship with the original host, but we identified several specific haplotypes common to an aphid origin. Ten distinct IGS haplotypes were detected in the IGS region, some of which were associated with aphid and whitefly origins. These result s suggest that the haplotype of rDNA RFLP analysis can be used for studying genetic diversity in V lecanii.