Possible role of heat shock protein 60 in reducing ischemic-reperfusion injury in canine pancreas grafts after preservation by the two-layer method

Citation
Y. Fujino et al., Possible role of heat shock protein 60 in reducing ischemic-reperfusion injury in canine pancreas grafts after preservation by the two-layer method, PANCREAS, 23(4), 2001, pp. 393-398
Citations number
24
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
PANCREAS
ISSN journal
0885-3177 → ACNP
Volume
23
Issue
4
Year of publication
2001
Pages
393 - 398
Database
ISI
SICI code
0885-3177(200111)23:4<393:PROHSP>2.0.ZU;2-B
Abstract
Introduction: Recently, results of the clinical application of the two-laye r method have shown the morphologic quality of the human pancreas grafts af ter reperfusion to be excellent, although ischemia-reperfusion injury is re lated to early graft loss in pancreas transplantation, However, some report s have indicated that heat shock proteins (HSPs) have important functions i n response to the stress-related events. Aim: To examine whether the two-layer method reduced ischemia-reperfusion i njury in a canine pancreas autotransplantation model by investigating the e xpression of HSPs. Methodology: There were three experimental groups in which dogs received se gmental autografts after preservation by the two-layer method using Univers ity of Wisconsin solution (UW) (group 1), simple storage in UW (group 2) fo r 24 hours, or no preservation (group 3). Results: In group 1, pancreatic tissue perfusions were high, and pancreatic exocrine functions were well preserved after 1, 2, and 4 hours of reperfus ion with low incidence of graft pancreatitis or vessel thrombosis compared with that in group 2. Moreover, ATP rapidly recovered, and HSP 60 was stron gly enhanced after reperfusion in group 1. On the other hand, ATP recovery and the enhancement of HSP 60 were weak after reperfusion in group 2. Conclusion: The two-layer method reduced ischemia-reperfusion injury compar ed with UW simple storage in canine pancreas autotransplantation with a str ong expression of HSP 60.