Y. Fujino et al., Possible role of heat shock protein 60 in reducing ischemic-reperfusion injury in canine pancreas grafts after preservation by the two-layer method, PANCREAS, 23(4), 2001, pp. 393-398
Introduction: Recently, results of the clinical application of the two-laye
r method have shown the morphologic quality of the human pancreas grafts af
ter reperfusion to be excellent, although ischemia-reperfusion injury is re
lated to early graft loss in pancreas transplantation, However, some report
s have indicated that heat shock proteins (HSPs) have important functions i
n response to the stress-related events.
Aim: To examine whether the two-layer method reduced ischemia-reperfusion i
njury in a canine pancreas autotransplantation model by investigating the e
xpression of HSPs.
Methodology: There were three experimental groups in which dogs received se
gmental autografts after preservation by the two-layer method using Univers
ity of Wisconsin solution (UW) (group 1), simple storage in UW (group 2) fo
r 24 hours, or no preservation (group 3).
Results: In group 1, pancreatic tissue perfusions were high, and pancreatic
exocrine functions were well preserved after 1, 2, and 4 hours of reperfus
ion with low incidence of graft pancreatitis or vessel thrombosis compared
with that in group 2. Moreover, ATP rapidly recovered, and HSP 60 was stron
gly enhanced after reperfusion in group 1. On the other hand, ATP recovery
and the enhancement of HSP 60 were weak after reperfusion in group 2.
Conclusion: The two-layer method reduced ischemia-reperfusion injury compar
ed with UW simple storage in canine pancreas autotransplantation with a str
ong expression of HSP 60.