Providing the basis for genomics in Lotus japonicus: the accessions Miyakojima and Gifu are appropriate crossing partners for genetic analyses

Citation
M. Kawaguchi et al., Providing the basis for genomics in Lotus japonicus: the accessions Miyakojima and Gifu are appropriate crossing partners for genetic analyses, MOL GENET G, 266(2), 2001, pp. 157-166
Citations number
34
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Molecular Biology & Genetics
Journal title
MOLECULAR GENETICS AND GENOMICS
ISSN journal
1617-4615 → ACNP
Volume
266
Issue
2
Year of publication
2001
Pages
157 - 166
Database
ISI
SICI code
1617-4615(200110)266:2<157:PTBFGI>2.0.ZU;2-7
Abstract
Lotus japonicus has attracted attention as a model plant legume for molecul ar genetic research, and several mutants defective in nodulation and mycorr hizal symbiosis have been developed from the standard accession Gifu B-129. However, as a model system, Gifu has long lacked an appropriate crossing p artner for use in various genetic analyses. In a search for an appropriate partner for Gifu, we have collected plants from 15 localities throughout Ja pan, and analyzed their levels of DNA polymorphism (also in comparison to t he African species L. filicaulis) by AFLP (Amplified Fragment Length Polymo rphism) combined with the use of a high-throughput electrophoretic screenin g system termed HEGS (High-efficiency genome scanning) developed by us, usi ng 31 primer pairs. Plants of the accession Miyakojima MG-20 showed the hig hest level of polymorphism relative to Gifu (over 4%). When HEGS is used fo r screening, this level is sufficient to permit systematic positional cloni ng of mutant genes. Segregation in the F-2 of the Gifu-derived symbiotic mu tations Ljsym70, Ljsym72, Ljsym74-1 (alb1-1) and Ljsym78-1 from a cross wit h Miyakojima was normal, while the ratios seen from a cross with L. filicau lis were distorted. Miyakojima displays several traits that distinguish it from other Japanese accessions: low concentrations of anthocyanin in the st em and petals, few trichomes, a more upright habit, broad leaflets and peta ls, and large black seeds. The first two traits, which are controlled by si ngle recessive genes, serve as useful markers for following mutant crosses.