Cytochemical observations on the relationship between lysosomes and phagosomes in kidney and liver by combined staining for acid phosphatase and intravenously injected horseradish peroxidase (Reprinted from J. Cell Biol, vol20, pg 497-507, 1964)

Citation
W. Straus et Ei. Christensen, Cytochemical observations on the relationship between lysosomes and phagosomes in kidney and liver by combined staining for acid phosphatase and intravenously injected horseradish peroxidase (Reprinted from J. Cell Biol, vol20, pg 497-507, 1964), J AM S NEPH, 12(11), 2001, pp. 2528-2537
Citations number
54
Language
INGLESE
art.tipo
Reprint
Categorie Soggetti
Urology & Nephrology","da verificare
Journal title
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
ISSN journal
1046-6673 → ACNP
Volume
12
Issue
11
Year of publication
2001
Pages
2528 - 2537
Database
ISI
SICI code
1046-6673(200111)12:11<2528:COOTRB>2.0.ZU;2-R
Abstract
After incubation of formalin-fixed, frozen sections of kidney and liver fro m peroxidase-treated rats in an azo dye medium for acid phosphatase, and af ter subsequent incubation of the same sections with benzidine, phagosomes w ere stained blue and lysosomes were stained red in the same cells. It was o bserved that newly formed phagosomes were separate from preexisting lysosom es in the tubule cells of the kidney and in the Kupffer cells of the liver at early periods after treatment with peroxidase. At later periods, the col or reactions for acid phosphatase and peroxidase occurred in the same granu les. The reaction of peroxidase decreased gradually and disappeared from th e phago-lysosomes after 2 to 3 days, whereas the reaction for acid phosphat ase persisted. In the liver, most of the injected protein was concentrated in large phagosomes located at the periphery of the cells lining the sinuso ids. The peribiliary lysosomes showed a relatively weak reaction for peroxi dase in the proximity of the portal veins. After pathological changes of pe rmeability, phagosomes and lysosomes lost their normal location and fused, in the interior of many liver cells, to form large vacuoles or spheres. The effects of a reduced load of peroxidase and the effects of the pretreatmen t with another protein (egg white) on the phago-lysosomes of the kidney wer e tested. The relationship of the fusion of phagosomes with lysosomes to th e size of normal and pathological phago-lysosomes was discussed.