Cellular distribution and function of soluble guanylyl cyclase in rat kidney and liver

Citation
F. Theilig et al., Cellular distribution and function of soluble guanylyl cyclase in rat kidney and liver, J AM S NEPH, 12(11), 2001, pp. 2209-2220
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Urology & Nephrology","da verificare
Journal title
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
ISSN journal
1046-6673 → ACNP
Volume
12
Issue
11
Year of publication
2001
Pages
2209 - 2220
Database
ISI
SICI code
1046-6673(200111)12:11<2209:CDAFOS>2.0.ZU;2-2
Abstract
Soluble guanylyl cyclase (sGC) catalyzes the biosynthesis of cGMP in respon se to binding Of L-arginine-derived nitric oxide (NO). Functionally, the NO -sGC-cGMP signaling pathway in kidney and liver has been associated with re gional hemodynamics and the regulation of glomerular parameters. The distri bution of the ubiquitous sGC isoform alpha1 beta1 sGC was studied with a no vel, highly specific antibody against the beta1 subunit. In parallel, the p resence of mRNA encoding both subunits was investigated by using in situ hy bridization and reverse transcription-PCR assays. The NO-induced, sGC-depen dent accumulation of cGMP in cytosolic extracts of tissues and cells was me asured in vitro. Renal glomerular arterioles, including the renin-producing granular cells, mesangium, and descending vasa recta, as well as cortical and medullary interstitial fibroblasts, expressed sGC. Stimulation of isola ted mesangial cells, renal fibroblasts, and hepatic Ito cells with a NO don or resulted in markedly increased cytosolic cGMP levels. This assessment of sGC expression and activity in vascular and interstitial cells of kidney a nd liver may have implications for understanding the role of local cGMP sig naling cascades.