A sialoglycopeptide was fractionated and purified on Sephadex G-25 and QAE-
Sephadex A-25. Its homogeneity was confirmed by reverse phase-HPLC and elec
trophoresis. It consisted of NeuNAc, Fuc, Gal, Man, GlcNAc in the ratio 1:1
:2:1:1 and Asp, Glu, Ser, Thr, Pro as major amino acids. Lysine was found t
o be the N-terminal amino acid residue. Antibodies raised to the glycopepti
de were immunogenic and were similar to blood group-A substance as determin
ed by immunodouble diffusion and hemagglutination techniques.