Purification of an antigenic glycopeptide from buffalo colostrum

Authors
Aparna, HS Salimath, PV
Citation
Hs. Aparna et Pv. Salimath, Purification of an antigenic glycopeptide from buffalo colostrum, J FD SCI M, 38(5), 2001, pp. 450-452
Citations number
27
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Food Science/Nutrition
Journal title
JOURNAL OF FOOD SCIENCE AND TECHNOLOGY-MYSORE
ISSN journal
0022-1155 → ACNP
Volume
38
Issue
5
Year of publication
2001
Pages
450 - 452
Database
ISI
SICI code
0022-1155(200109/10)38:5<450:POAAGF>2.0.ZU;2-2
Abstract
A sialoglycopeptide was fractionated and purified on Sephadex G-25 and QAE- Sephadex A-25. Its homogeneity was confirmed by reverse phase-HPLC and elec trophoresis. It consisted of NeuNAc, Fuc, Gal, Man, GlcNAc in the ratio 1:1 :2:1:1 and Asp, Glu, Ser, Thr, Pro as major amino acids. Lysine was found t o be the N-terminal amino acid residue. Antibodies raised to the glycopepti de were immunogenic and were similar to blood group-A substance as determin ed by immunodouble diffusion and hemagglutination techniques.