High fat diet enhances colonic cell proliferation and carcinogenesis in rats by elevating serum leptin

Citation
Zh. Liu et al., High fat diet enhances colonic cell proliferation and carcinogenesis in rats by elevating serum leptin, INT J ONCOL, 19(5), 2001, pp. 1009-1014
Citations number
43
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Onconogenesis & Cancer Research
Journal title
INTERNATIONAL JOURNAL OF ONCOLOGY
ISSN journal
1019-6439 → ACNP
Volume
19
Issue
5
Year of publication
2001
Pages
1009 - 1014
Database
ISI
SICI code
1019-6439(200111)19:5<1009:HFDECC>2.0.ZU;2-S
Abstract
We postulated that high fat diet enhances colon cell proliferation and carc inogenesis by elevating serum leptin. To examine this possibility, the pres ent study was conducted to investigate the effect of leptin on the growth o f human colon cancer cells (HT29) and the relationship between serum leptin and colon cell proliferation and aberrant crypt foci (ACF) in the 1,2-dime thylhydrazine-treated rats fed graded levels of dietary fat for 28 days. In cell culture experiments, leptin stimulated the growth and proliferation ( BrdU incorporation) of colon cancer cells and the expression of c-fos prote in. In the in vivo experiments, an elevation of dietary fat caused higher s erum leptin and adipose-tissue weight. Colonic cell proliferation (BrdU inc orporation), c-fos protein expression and ACF were elevated with increasing dietary fat. There was a significant correlation between serum concentrati on of leptin and colon cell proliferation and ACF. The results suggest that the enhancement of colon cell proliferation and carcinogenesis by high fat diet is mediated through elevating serum leptin.