Influence of melatonin on basal and gonadotropin-stimulated progesterone and estradiol secretion of cultured human granulosa cells and in the superfused granulosa cell system

Citation
J. Bodis et al., Influence of melatonin on basal and gonadotropin-stimulated progesterone and estradiol secretion of cultured human granulosa cells and in the superfused granulosa cell system, GYNECOL OBS, 52(3), 2001, pp. 198-202
Citations number
8
Language
INGLESE
art.tipo
Article
Categorie Soggetti
da verificare
Journal title
GYNECOLOGIC AND OBSTETRIC INVESTIGATION
ISSN journal
0378-7346 → ACNP
Volume
52
Issue
3
Year of publication
2001
Pages
198 - 202
Database
ISI
SICI code
0378-7346(2001)52:3<198:IOMOBA>2.0.ZU;2-U
Abstract
The aim of this study was to explore the direct action of melatonin (Me) on basal and gonadotropin-stimulated progesterone (PG) and estradiol (E-2) se cretion of human granulosa cells (GCs) cultured in serum-free medium and in a superfused GC system. Human GCs were isolated from preovulatory follicul ar fluid aspirated from 34 women undergoing in vitro fertilization at the U niversity Women's Hospital of Tubingen. PG and E-2 production was measured in the presence and absence of Me, propranolol, LH or FSH using radioimmuno assay. Statistical analysis of the data was performed by ANOVA and Newman-K euls test. Me stimulated E-2 secretion in a dose-dependent manner. Proprano lol did not cause any change in E-2 secretion, and when given with Me, it o nly partially blocked but could not entirely prevent E-2 output. There was no statistically significant effect of Me on PG production when Me was admi nistered at concentrations between 10(-4) and 10(-8) M. However, at 10(-3) M Me significantly suppressed PG output of granulosa cells. LH and FSH sign ificantly stimulated the secretion of both steroid hormones. Me significant ly reduced LH- and FSH-induced E-2 secretion, as well as LH-stimulated PG o utput, while it caused only a slight, yet significant decrease in PG secret ion. In the superfused GC system, FSH and LH resulted in a significant stim ulatory effect on PG release. Me did not modify the stimulatory effect of F SH on PG, while it caused some delay in LH-stimulated PG release. Propranol ol and Me had no stimulatory effect on PG release. On the basis of our resu lts we suggest that Me has a direct modulatory effect on basal E-2 and gona dotropin-stimulated E-2 and PG secretion of human GCs. The observed effect may play a physiological role in the regulation of GC function during the m enstrual cycle. Copyright (C) 2001 S. Karger AG, Basel.