Dextran sodium sulfate-induced colitis in germ-free IQI/Jic mice

Citation
S. Kitajima et al., Dextran sodium sulfate-induced colitis in germ-free IQI/Jic mice, EXP ANIM, 50(5), 2001, pp. 387-395
Citations number
29
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Animal Sciences
Journal title
EXPERIMENTAL ANIMALS
ISSN journal
1341-1357 → ACNP
Volume
50
Issue
5
Year of publication
2001
Pages
387 - 395
Database
ISI
SICI code
1341-1357(200110)50:5<387:DSSCIG>2.0.ZU;2-T
Abstract
This study presents a histological examination of dextran sodium sulfate (D SS)induced colitis in germ-free (GF) mice. A comparison of the pathology be tween GF and conventionalized mice (CVz) was made to determine the role tha t intestinal microflora play in DSS-induced colitis. To induce colitis, GF and CVz IQI/Jic mice were given either 5% or 1% DSS orally. Administration of 5% DSS, a common concentration used to induce colitis in mice, caused gr oss rectal bleeding and a marked decrease in hematocrit as early as day one in GF mice. These mice died on day three due to massive bleeding into the intestinal lumen. In contrast, CVz mice did not die during the seven-day ex perimental period. Histopathological examination three days after administr ation of 5% DSS did not reveal any colitis lesions in GF mice, but CVz mice had developed moderate colitis in the large intestine. Administration of a low concentration of DSS (1%), which only induces mild basal crypt loss in CVz mice, caused severe colitis in the distal colon in GF mice, and they d ied on day 14. These data suggest that intestinal microflora are not necess ary for the induction of colitis. Furthermore, DSS may be highly toxic to G F mice, and when given at a concentration of 5% it causes massive bleeding into the intestinal lumen resulting in death prior to development of coliti s.