Rapid detection of several mycobacterial species using a polymerase chain reaction reverse hybridisation assay

Citation
E. Padilla et al., Rapid detection of several mycobacterial species using a polymerase chain reaction reverse hybridisation assay, EUR J CL M, 20(9), 2001, pp. 661-665
Citations number
14
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Microbiology
Journal title
EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
ISSN journal
0934-9723 → ACNP
Volume
20
Issue
9
Year of publication
2001
Pages
661 - 665
Database
ISI
SICI code
0934-9723(200109)20:9<661:RDOSMS>2.0.ZU;2-Z
Abstract
The ability of a poly merase-chain-reaction-based technique combined with a reverse hybridisation line-probe assay to detect and identify eight of the most clinically significant mycobacterial species directly from cultures i n liquid medium was evaluated. The line-probe assay allows simultaneous ide ntification of eight different mycobacterial species. Ninety-seven mycobact erial strains belonging to 22 different species were evaluated. All strains were previously inoculated into MB/BacT bottles (Organon Teknika, USA). Th e sensitivity and specificity of the test when applied on positive MB/BacT liquid cultures containing isolates from mycobacterial species included as specific probes on the line-probe assay strip was 100% and 100%, respective ly. These results further support the potential clinical usefulness of this technique in the diagnosis of mycobacterial infections.