Pulse modulation - A novel approach to gradient-based flow injection techniques

Citation
A. Herbelin et J. Ruzicka, Pulse modulation - A novel approach to gradient-based flow injection techniques, COLL CZECH, 66(8), 2001, pp. 1219-1237
Citations number
17
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Chemistry
Journal title
COLLECTION OF CZECHOSLOVAK CHEMICAL COMMUNICATIONS
ISSN journal
0010-0765 → ACNP
Volume
66
Issue
8
Year of publication
2001
Pages
1219 - 1237
Database
ISI
SICI code
0010-0765(200108)66:8<1219:PM-ANA>2.0.ZU;2-O
Abstract
Development of a novel system for generation of gradients in flow injection analysis by pulse modulation is described. These user-selectable gradients are created by computercontrolled mixing of two solutions with a total vol ume as low as 75 muI and can be delivered under incremental or continuous f low conditions. Applications such as automated, single-standard instrument calibration are expected to benefit from high-precision linear gradients (r (2) = 0.99989, n = 55). Gradient methods in biochemisty and immunology such as kinetic measurement of biomolecular interactions will benefit from the small volume of these gradients, especially for analytes with limited avail ability.