Human liver disease decreases methacrylyl-CoA hydratase and beta-hydroxyisobutyryl-CoA hydrolase activities in valine catabolism

Citation
K. Ishigure et al., Human liver disease decreases methacrylyl-CoA hydratase and beta-hydroxyisobutyryl-CoA hydrolase activities in valine catabolism, CLIN CHIM A, 312(1-2), 2001, pp. 115-121
Citations number
16
Language
INGLESE
art.tipo
Article
Categorie Soggetti
Medical Research Diagnosis & Treatment
Journal title
CLINICA CHIMICA ACTA
ISSN journal
0009-8981 → ACNP
Volume
312
Issue
1-2
Year of publication
2001
Pages
115 - 121
Database
ISI
SICI code
0009-8981(200110)312:1-2<115:HLDDMH>2.0.ZU;2-2
Abstract
Background: Methacrylyl-coenzyme A (MC-CoA) hydratase arid beta -hydroxyiso butyryl-coenzyme A (HIB-CoA) hydrolase are key enzymes regulating the toxic concentration of MC-CoA generated in valine catabolism. Materials and Meth ods: We studied the activities and mRNA expression levels of these enzymes in normal human livers and in human livers with chronic hepatitis, cirrhosi s, or hepatocellular carcinoma. Results: The activities of both enzymes wer e significantly lower by 36% to 46% in livers with cirrhosis or hepatocellu lar carcinoma compared with normals, suggesting a decrease in the capabilit y of detoxifying MC-CoA with these diseases. The mRNA levels for both enzym es measured by quantitative polymerase chain reaction were significantly in creased in livers with cirrhosis, but were not altered in those with chroni c hepatitis or hepatocellular carcinoma when compared with normal livers. C onclusion: Our results suggest that low levels of these enzyme activities i n livers with cirrhosis or hepatocellular carcinoma are the result of postt ranscriptional regulation in the damaged liver. (C) 2001 Elsevier Science B .V. All rights reserved.