Homology-dependent gene silencing mechanisms in fungi

Authors
Citation
C. Cogoni, Homology-dependent gene silencing mechanisms in fungi, ANN R MICRO, 55, 2001, pp. 381-406
Citations number
154
Language
INGLESE
art.tipo
Review
Categorie Soggetti
Microbiology
Journal title
ANNUAL REVIEW OF MICROBIOLOGY
ISSN journal
0066-4227 → ACNP
Volume
55
Year of publication
2001
Pages
381 - 406
Database
ISI
SICI code
0066-4227(2001)55:<381:HGSMIF>2.0.ZU;2-R
Abstract
Homology-dependent gene silencing (HDGS) is a ubiquitous phenomenon among f ungi, plants, and animals. Gene silencing can be triggered and can affect a rtificially introduced nucleic acid molecules, both DNA and RNA, and/or can act on endogenous duplicated sequences. Although the various HDGS phenomen a may be related each other, probably deriving from an ancestral defense me chanism, relevant differences do exist between different HDGS mechanisms. E specially in fungi, a variety of HDGS phenomena have been uncovered during the past 10 years: Gene inactivation of duplicated sequences can be achieve d either through DNA-methylation and block of transcription or through sequ ence-specific degradation of mRNA. Moreover, duplicated sequences can also be specifically mutagenized. Studying HDGS in fungi gives us the opportunit y to study such complex mechanisms in relatively simple organisms in which both genetic and biochemical approaches can be easily used.